PATRIC has 2 Mass Spectrometry experiment(s) for Brucella
Direct evidence 
| Experiment Type | Experiment Overview | Experiment Description | Experiment Design | Experiment Results |
|---|---|---|---|---|
| Mass Spectrometry | CAPRION_01 (Links to PRC) |
To gain insight into the basis of Brucella virulence, which is linked to the outer membrane (OM) and requires a functional BvrR/BvrS, we have performed a highresolution quantitative proteomic study of OMF from virulent data B. abortus, bvrR-, and bvrS- avirulent mutants, and a reconstituted virulent bvrR+ (bvrR-/pbvrR+) strain. (Pathogen data) | The purified OMF were digested by proteolysis to derive peptides which were separated by liquid-phase chromatography and analyzed using quantitative, label-free, mass spectrometry-based methods. | View |
| Mass Spectrometry | CAPRION_02 (Links to PRC) |
Characterization of host and pathogen proteins affected by Brucella abortus infection. (Host and Pathogen data) | Developing methods for solubilizing cell envelope and whole bacteria protein | View |
Host
Direct evidence
| Experiment Type | Experiment Overview | Experiment Description | Experiment Design |
|---|---|---|---|
| Microarray | GSE5202 (Links to GEO) |
Expression data from B. melitensis infected mouse macrophages | Murine J774.A1 macrophage cells were infected with B. melitensis strain 16M at a MOI of 200:1. Brucella cultures derived from different Brucella colonies were used to infect different groups of macrophage cells to reflect independent infections. Following 4 h incubation, the cells were washed with PBS and treated with 50 ug/ml gentamicin to kill extracellular Brucella. At 0 h (no Brucella infection), 4 h, 24 h, and 48 h post-infection, cells were individually collected, and total RNA was isolated. The Affymetrix mouse GeneChip 430 2.0 array was used for microarray hybridization. Microarray intensity data were obtained by using Affymetrix GCOS software and further analyzed by GeneSpring and other software programs. |
